紫外线-B诱导桑叶中次生代谢产物变化及其差异蛋白质组学研究

虞姣姣，胡瑾，管祺杰，刘京龙，高成成，谷希达，朱玮

中国药学杂志 ›› 2016, Vol. 51 ›› Issue (14) : 1191-1196.

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中国药学杂志
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中国药学杂志 ›› 2016, Vol. 51 ›› Issue (14) : 1191-1196. DOI: 10.11669/cpj.2016.14.010
“一带一路”沿线国家传统药物研究专栏

紫外线-B诱导桑叶中次生代谢产物变化及其差异蛋白质组学研究

  • 虞姣姣，胡瑾，管祺杰，刘京龙，高成成，谷希达，朱玮*
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Changes of Secondary Metabolites and Differential Proteomics in Mulberry Leaves under UV-B Induction

  • YU Jiao-jiao, HU Jin, GUAN Qi-jie, LIU Jing-long, GAO Cheng-cheng, GU Xi-da, ZHU Wei*
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摘要

目的 考察紫外线-B(UV-B)诱导下不同暗培养时间对桑叶中两种次生代谢产物的影响及诱导前后差异蛋白组学研究。方法 以高效液相色谱法建立UV-B诱导前后桑叶甲醇提取物的指纹图谱，并对其中的两种次生代谢产物进行定量分析;利用透射电镜观察UV-B诱导下桑叶亚细胞结构的变化;以双向电泳技术对UV-B诱导前后的桑叶进行差异蛋白质组学分析。结果 在UV-B诱导下，桑叶的亚细胞结构受到严重破坏，桑叶中桑辛素N和查桑酮的含量随着暗培养时间的增加而增加，分别在暗培养36和40 h达到最大值;差异蛋白质组学结果发现黄酮合成途径中查尔酮合酶的表达量显著升高。结论 UV-B诱导联合暗培养的胁迫方式，改变了桑叶体内次生代谢过程特别是黄酮生物合成途径，致使桑辛素N和查桑酮的大量合成。

Abstract

OBJECTIVE To investigate the influence of ultraviolet B(UV-B) induction on the content of two kinds of secondary metabolites in mulberry leaves and differential proteomics under UV-B induction. METHODS The HPLC chromatograms of secondary metabolites in leaves before and after UV-B induction were established and quantitative analysis of two kinds of secondary metabolites was performed. The subcellular structure of mulberry leaves under UV-B induction was observed using transmission electron microscopy. Proteomic analysis of mulberry leaves was performed using two-dimensonal electrophoresis(2-DE). RESULTS The subcellular structure of mulberry leaves was destroyed under UV-B induction. The contents of moracin N and chalcomoracin in mulberry leaves were increased with the dark incubation time and they maximized at 36 and 40 h, respectively. The result of proteomics showed that the abundance of chalcone synthase(CHS) which is the key enzyme of flavonoid biosynthesis pathway was increased significantly. CONCLUSION The secondary metabolism especially for flavonoids biosynthesis in mulberry leaves is changed under UV-B induction and dark incubation, which result in the increased moracin N and chalcomarcin.

关键词

紫外线-B诱导 / 桑叶 / 次生代谢 / 蛋白质组学

Key words

UV-B induction / mulberry leaf / secondary metabolism / proteomics

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虞姣姣，胡瑾，管祺杰，刘京龙，高成成，谷希达，朱玮. 紫外线-B诱导桑叶中次生代谢产物变化及其差异蛋白质组学研究[J]. 中国药学杂志, 2016, 51(14): 1191-1196 https://doi.org/10.11669/cpj.2016.14.010
YU Jiao-jiao, HU Jin, GUAN Qi-jie, LIU Jing-long, GAO Cheng-cheng, GU Xi-da, ZHU Wei. Changes of Secondary Metabolites and Differential Proteomics in Mulberry Leaves under UV-B Induction[J]. Chinese Pharmaceutical Journal, 2016, 51(14): 1191-1196 https://doi.org/10.11669/cpj.2016.14.010
中图分类号： R284   

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基金

教育部科学技术研究项目(113037A);浙江省自然科学基金资助项目(Z14H280001)
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